Sua Myong is an associate professor in the Department of Biophysics at Johns Hopkins University. Her research focuses on quantitative analysis of gene expression in single molecule and single cell. Her research interests involve developing and applying single molecule and single cell approaches to acquire quantitative understanding of biology and to find ways to improve human medicine. Single molecule platform offers an exquisite capability to detect molecular interactions and dynamics in real time. They devise sequential experimental stations that measures reactions at the molecular, biophysical and cellular levels. The Myong laboratory consist of scientists trained in molecular biology, physics, physical chemistry, bioengineering and chemical engineering, which is an ideal team setting for carrying out a highly interdisciplinary research.
The current directions of their research include the following. (i) DNA recombination and repair in which they study proteins involved in homologous recombination and trinucleotide repeat processing (collaborate with Patrick Sung and Timothy Lohman; Nat Comms 2013); (ii) Telomere and telomerase processing where they investigate the Shelterin protein components and their interaction with telomerase (collaborate with Patricia Opresko; Structure 2012, Structure 2014, Scientific Reports 2014); (iii) RNA interference mechanism for which they devised stepwise testing platforms to quantitatively analyze RNAi silencing efficiency (collaborate with Jennifer Doudna; PNAS, 2013, NAR 2014) (iv) Ribonucleoprotein assembly and dynamics where they seek to understand the role of RNA helicases in nucleating and forming granules in vitro and in cells (collaborate with Clifford Brangwynne and Christian Eckmann;PNAS, 2015); (v) G quadruplex (GQ) DNA mediated gene regulation where they investigate the role of GQ in altering transcription and translation (NAR 2014; NAR 2015).
On a technical front, they have pioneered a new method for single molecule fluorescence imaging termed PIFE for protein induced fluorescence enhancement (PNAS 2011). This method offers a unique opportunity of performing single molecule experiment without having to fluorescently label the protein under study, thus expanding the capability of the current single molecule imaging. Recently, they established a real-time single molecule assay for detecting telomerase extension activity (Scientific Report, 2015) and GQ conformation probing assay by induced fluorescence of GQ ligands (NAR, 2015) They expect the outcome of our studies to uncover molecular details that govern cellular responses. This can open a new way of drug targeting, provide drug screening platforms and help optimize drug efficiency. In addition, their capability to perform single cell measurements will lead to better understanding about the cell to cell heterogeneity, which poses a challenging problem in research and biomedicine.
Scott Edwards is Alexander Agassiz Professor of Zoology and Curator of Ornithology in the Museum of Comparative Zoology at Harvard University. He came to Harvard in December 2003 after serving as a faculty for 9 years in the Zoology Department and the Burke Museum at the University of Washington, Seattle. His research focuses on diverse aspects of avian biology, including evolutionary history and biogeography, disease ecology, population genetics and comparative genomics. He has conducted fieldwork in phylogeography in Australia since 1987 and conducted some of the first phylogeographic analyses based on DNA sequencing. He did a postdoctoral fellowship in immunogenetics at the University of Florida and gained experience with studying the major histocompatibility complex (MHC) of birds, an important gene complex for interactions of birds and infectious diseases, pathogens and mate choice. An important system for studying these issues is the ongoing epizootic involving House Finches (Haemorhous mexicanus) and the bacterial pathogen Mycoplasma gallisepticum. His work on the MHC led him to study the large-scale structure of the avian genome and informed his current interest in using comparative genomics to study the genetic basis of phenotypic innovation in birds. In the last 10 years Dr. Edwards has helped develop novel methods for estimating phylogenetic trees from multilocus DNA sequence data. His recent work uses comparative genomics in diverse contexts to study macroevolutionary patterns in birds, including the origin of feathers and the evolution of flightlessness.
From 2013-2015 Scott served as Division Director of the Division of Biological Infrastructure at the US National Science Foundation, where he oversaw a staff of 22, an annual research budget of $120M (USD), and managed funding programs focused on undergraduate research, postdoctoral fellowships, natural history collections and field stations, and cyber- and other infrastructure for all areas of biology, from molecular to ecosystem science. He has served as President of three international scientific societies based in the US: the Society for the Study of Evolution, the Society of Systematic Biologists, and the American Genetic Association, each of which publishes a scientific journal and has memberships ranging from 500 – 2500 scientists and students. He has served on the National Geographic’s Committee for Research and Exploration, the Senior Advisory Boards of the NSF-funded US National Evolutionary Synthesis Center (NESCent) and the National Institute for Mathematical and Biological Synthesis (NIMBioS), and on the Advisory Boards of the National Museum of Natural History at the Smithsonian and the Cornell Lab of Ornithology. He oversees a program funded by the National Science Foundation to increase the diversity of undergraduates in evolutionary biology and biodiversity science. He is a member of the American Academy of Arts and Sciences (2009), a Fellow of the American Association of the Advancement of Science (2009), and a member the National Academy of Sciences (2015).